Biomod/2011/TUM/TNT/LabbookA/list TEM pictures

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       TUM NanU - Home

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TEM

All dilutions of the structures were made with FOB20. For the calculation of the intercalator concentration read more in the corresponding labbook entry. Due to a limitation in webspace we only uploaded a selection of 6 pictures for each grid.

BIOMOD1 Grid Box

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grid number	prepared	annotations	observed	annotation

B2	sep. 16th	<a href="http://openwetware.org/images/5/56/20110921_theU_BM2.png">BM2 1:5 control without DNA-binders</a>	sep. 21th	fine grid
B5	sep. 16th	<a href="http://openwetware.org/images/9/99/20110921_theU_BM2_100uM_Spermine.png">BM2 1:5 with 100 µM spermine</a>	sep. 21th	structures accumulated
C2	sep. 16th	<a href="http://openwetware.org/images/3/37/20110921_theU_BM2_25uM_EtBr.png">BM2 1:5 with 25 µM ethidium bromide</a>	sep. 21th	massive deformation of the structures
C3	sep. 16th	<a href="http://openwetware.org/images/3/32/20110921_theU_BM2_75uM_EtBr.png">BM2 1:5 with 75 µM ethidium bromide</a>	sep. 21th	broken grid
C4	sep. 16th	<a href="http://openwetware.org/images/a/af/20110921_theU_BM2_250uM_EtBr.png">BM2 1:5 with 250 µM ethidium bromide</a>	sep. 21th	destroyed structures, not enough structures

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BIOMOD2 Grid Box

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grid number	prepared	annotations	observed	annotation

A1	oct. 5th	<a href="http://openwetware.org/images/c/c3/20111007_theU_BM2_1_34UM_SPERMIN_A1.png">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</a>	oct. 7th	good stain, slightly to low dilution, 62 images
A2	oct. 5th	<a href="http://openwetware.org/images/7/76/20111007_theU_BM2_1_34um_spermin_a2.png">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</a>	oct. 7th	good stain, slightly to low dilution, 63 images
A3	oct. 5th	<a href="http://openwetware.org/images/b/b9/20111007_theU_BM2_1_34um_spermin_a3.png">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</a>	oct. 7th	good stain, slightly to low dilution, 60 images
A4	oct. 5th	<a href="http://openwetware.org/images/9/9f/20111007_theU_BM2_0_42UMSPERMIN_A4.png">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</a>	oct. 7th	good stain, slightly to low dilution, 64 images
A5	oct. 5th	<a href="http://openwetware.org/images/7/7e/20111007_theU_BM2_0_24uM_Spermin_a5.png">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</a>	oct. 7th	good stain, slightly to low dilution, 63 images
A6	oct. 20th	BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)	oct. 28st	no origamis on the grid, no images
A7	oct. 20th	BM2 1:25 with 144 nM DAPI (corresponding to one molecule every 21bp)	oct. 27th	no origamis on the grid, no images
A8	oct. 20th	BM2 1:25 with 144 nM DAPI (corresponding to one molecule every 21bp)	oct. 28th	no origamis on the grid, no images
A9	oct. 20th	<a href="http://openwetware.org/images/b/b1/20111021_theU_BM2_2A9_control.png">BM2 1:25 without any DNA binders</a>	oct. 21th	good stain and dilution, 40 images
A10	oct. 20th	<a href="http://openwetware.org/images/5/59/20111021_theU_BM2_2A10_control.png">BM2 1:25 without any DNA binders</a>	oct. 21th	good stain and dilution, 60 images

B1	oct. 5th	<a href="http://openwetware.org/images/d/d0/20111012_theU_BM2_0_42um_Spermin_b1.png">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</a>	oct. 12th	grid was fine, 60 images
B2	oct. 10th	<a href="http://openwetware.org/images/d/dc/20111012_theU_BM2_2_27um_Etbr_b2.png">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</a>	oct. 12th	too high dilution, 25 images
B3	oct. 10th	<a href="http://openwetware.org/images/7/7c/20111013_theU_BM2_2.27uM_EtBr_B3.png">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</a>	oct. 13th	good stain and structure concentration, 36 images
B4	oct. 10th	<a href="http://openwetware.org/images/b/b5/20111014_theU_BM2_2.27uM_EtBr_B4.png">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</a>	oct. 14th	good stain but slightly to low structure concentration, 35 images
B5	oct. 10th	BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)
B6	oct. 20th	<a href="http://openwetware.org/images/0/0a/20111021_theU_BM2_2B6_control.png">BM2 without any DNA binders, 1:25</a>	oct. 21th	good stain and dilution, 66 images
B7	oct. 20th	<a href="http://openwetware.org/images/9/9a/20111021_theU_BM2_2B7_control.png">BM2 without any DNA binders, 1:25</a>	oct. 21th	good stain and dilution, 80 images
B8	oct. 20th	<a href="http://openwetware.org/images/3/36/20111020_theU_BM24_2B8.png">BM24 15_65 1:25 without any DNA binders</a>	oct. 20th	bad stain and to high dilution
B9	oct. 20th	BM24 2DH3ML 1:25 without any DNA binders	oct. 20th	bad stain, no structures on the grid, 3 images
B10	oct. 21th	<a href="http://openwetware.org/images/b/ba/20111021_theU_BM24_2B10_15_65.png">BM24 15_65 1:20 without any DNA binders</a>	oct. 21th	good stain but slightly to high dilution, 61 images

C1	oct. 10th	<a href="http://openwetware.org/images/3/33/20111014_theU_BM2_0.69uM_EtBr_C1.png">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</a>	oct. 14th	good stain but dilution slightly to low, 32 images
C2	oct. 10th	<a href="http://openwetware.org/images/4/44/20111014_theU_BM2_0.69uM_EtBr_C2.png">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</a>	oct. 14th	good stain but dilution slightly to low, 31 images
C3	oct. 10th	BM2 1:40 without any DNA binders
C4	oct. 10th	BM2 1:40 without any DNA binders
C5	oct. 10th	BM2 1:40 without any DNA binders
C6	oct. 21th	<a href="http://openwetware.org/images/8/84/20111021_theU_BM24_2C6_15_65.pnghttp://openwetware.org/images/8/84/20111021_theU_BM24_2C6_15_65.png">BM24 15_65 dilution 1:20 without any DNA binders</a>	oct. 21th	stain was bad and to high dilution, 17 images
C7	oct. 21th	<a href="http://openwetware.org/images/8/89/20111021_theU_BM24_2C7_2DH3ML.png">BM24 2DH3ML dilution 1:20 without any DNA binders </a>	oct. 21th	stain was good but dilution slightly to high, 70 images
C8	oct. 21th	<a href="http://openwetware.org/images/2/2b/20111021_theU_BM24_2C8_2D3HML.png">BM24 2DH3ML 1:20 without any DNA binders</a>	oct. 21th	stain was good but dilution slightly to high
C9	oct. 27th	<a href="http://openwetware.org/images/7/74/20111027_theU_BM2_2C9_control.png">BM2 1:25 without any DNA binders</a>	oct. 27	good stain but to high dilution, 60 images
C10	oct. 27th	<a href="http://openwetware.org/images/3/31/20111027_theU_BM2_2C10_control059.png">BM2 1:25 without any DNA binders</a>	oct. 27th	fine grid, 60 images

D1	oct. 12th	<a href="http://openwetware.org/images/3/38/20111012_theU_BM2_2_4_um_Etbr_d1.png">BM2 1:25 with 2.4 µM ethidium bromide (corresponding to one molecule every 7bp)</a>	oct. 12th	good stain and dilution, 74 images
D2	oct. 12th	<a href="http://openwetware.org/images/0/0c/20111012_theU_BM2_0_74um_EtBr_d2.png">BM2 1:25 with 0.74 µM ethidium bromide (corresponding to one molecule every 21bp)</a>	oct. 12th	good stain and dilution, 89 images, chosen for class average
D3	oct. 12th	<a href="http://openwetware.org/images/0/09/20111012_theU_BM2_control_d3.png">BM2 1:25without any DNA binders </a>	oct. 12th	good stain and dilution, 50 images
D4	oct. 14th	<a href="http://openwetware.org/images/5/5c/20111020_theU_BM2_2D4_432nM_DAPI.png">BM2 1:25 with 432 µM DAPI (corresponding to one molecule every 7bp)</a>	oct. 20th	good stain and structur concentration but cluster, 62 images
D5	oct. 14th	<a href="http://openwetware.org/images/1/18/20111020_theU_2d5_144nM_dapi.png">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</a>	oct. 20th	stain and dilution was good, cluster, 61 images
D6	oct. 27th	<a href="http://openwetware.org/images/3/32/20111027_theU_BM24_2D6_control.png">BM24 (15_65), 1:20 diluted with FOB20</a>	oct. 27th	good stain but slightly to high dilution, 22 images
D7	oct. 27th	lost the grid before sample preparation
D8	oct. 27th	<a href="http://openwetware.org/images/9/9d/20111027_theU_BM24_2D8_control024.png">BM24 (15_65) 1:10 without any DNA binders</a>	oct. 27th	low sample concentration, 24 images
D9	oct. 27th	BM24 (15_65) 1:10 without any DNA binders		stain was good but dilution slightly to low
D10	oct. 28st	<a href="http://openwetware.org/images/d/d1/20111028_theU_BM24_2D10_control.png">BM24 (15_65) 1:5 without any DNA binders</a>	oct. 28st	better sample concentration than D6 and D8, 60 images

E1	oct. 20th	<a href="http://openwetware.org/images/1/18/20111101_theU_Bm2_2e1_2_4um_etbr.png">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</a>	oct. 26th	good dilution, bad staining, 49 images
E2	oct. 20th	<a href="http://openwetware.org/images/a/a7/20111026_theU_BM2_2E2_2.4uM_EtBr049.png">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</a>	oct. 26th	good dilution but strong background, 50 images
E3	oct. 20th	<a href="http://openwetware.org/images/f/f9/20111026_theU_BM2_2E3_0.74uM_EtBr059.png">BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)</a>	oct. 26th	fine grid, 60 images
E4	oct. 20th	<a href="http://openwetware.org/images/e/ed/20111026_theU_BM2_2E4_0.74uM_EtBr049.png">BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)</a>	oct. 26th	fine grid, 50 images
E5	oct. 20th	<a href="http://openwetware.org/images/1/14/20111026_theU_BM2_2E5_432nM_DAPI.png">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</a>	oct. 26th	good dilution but weak stain, 60 images
E6	oct. 28st	<a href="http://openwetware.org/images/9/94/20111028_theU_BM24_E6_control.png">BM24 (15_65), 1:5</a>	oct. 28st	fine grid, 413 images
E7	oct. 31th	<a href="http://openwetware.org/images/6/69/20111031_theU_B2_6.7uM_spermine_2E7.png">BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)</a>	oct. 31th	grid was fine, 112 images
E8	oct. 31th	<a href="http://openwetware.org/images/3/37/20111031_theU_B2_6.7uM_spermine_2E8.png">BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)</a>	oct. 31th	excellent grid, 80 images
E9	nov. 1st	<a href="http://openwetware.org/images/e/e7/20111101_theU_B2_12_1umSpermin_2E9.png">BM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)</a>	nov. 1st	good grid, 111 images
E10	nov. 1st	<a href="http://openwetware.org/images/a/ae/20111101_theU_B2_12_1umSpermin_2E10.png">BM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)</a>	nov. 1st	fine grid, 131 images

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Biomod/2011/TUM/TNT/LabbookA/list TEM pictures

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