Biomod/2011/TUM/TNT/LabbookA/list TEM pictures
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TEM
All dilutions of the structures were made with FOB20. For the calculation of the intercalator concentration read more in the corresponding labbook entry. Due to a limitation in webspace we only uploaded a selection of 6 pictures for each grid.
Contents |
BIOMOD1 Grid Box
| grid number | prepared | annotations | observed | annotation |
| B2 | sep. 16th | BM2 1:5 control without DNA-binders | sep. 21th | fine grid |
| B5 | sep. 16th | BM2 1:5 with 100 µM spermine | sep. 21th | structures accumulated |
| C2 | sep. 16th | BM2 1:5 with 25 µM ethidium bromide | sep. 21th | massive deformation of the structures |
| C3 | sep. 16th | BM2 1:5 with 75 µM ethidium bromide | sep. 21th | broken grid |
| C4 | sep. 16th | BM2 1:5 with 250 µM ethidium bromide | sep. 21th | destroyed structures, not enough structures |
BIOMOD2 Grid Box
| grid number | prepared | annotations | observed | annotation |
| A1 | oct. 5th | BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp) | oct. 7th | good stain, slightly to low dilution, 62 images |
| A2 | oct. 5th | BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp) | oct. 7th | good stain, slightly to low dilution, 63 images |
| A3 | oct. 5th | BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp) | oct. 7th | good stain, slightly to low dilution, 60 images |
| A4 | oct. 5th | BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp) | oct. 7th | good stain, slightly to low dilution, 64 images |
| A5 | oct. 5th | BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp) | oct. 7th | good stain, slightly to low dilution, 63 images |
| A6 | oct. 20th | BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp) | oct. 28st | no origamis on the grid, no images |
| A7 | oct. 20th | BM2 1:25 with 144 nM DAPI (corresponding to one molecule every 21bp) | oct. 27th | no origamis on the grid, no images |
| A8 | oct. 20th | BM2 1:25 with 144 nM DAPI (corresponding to one molecule every 21bp) | oct. 28th | no origamis on the grid, no images |
| A9 | oct. 20th | BM2 1:25 without any DNA binders | oct. 21th | good stain and dilution, 40 images |
| A10 | oct. 20th | BM2 1:25 without any DNA binders | oct. 21th | good stain and dilution, 60 images |
| B1 | oct. 5th | BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp) | oct. 12th | grid was fine, 60 images |
| B2 | oct. 10th | BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp) | oct. 12th | too high dilution, 25 images |
| B3 | oct. 10th | BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp) | oct. 13th | good stain and structure concentration, 36 images |
| B4 | oct. 10th | BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp) | oct. 14th | good stain but slightly to low structure concentration, 35 images |
| B5 | oct. 10th | BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp) | ||
| B6 | oct. 20th | BM2 without any DNA binders, 1:25 | oct. 21th | good stain and dilution, 66 images |
| B7 | oct. 20th | BM2 without any DNA binders, 1:25 | oct. 21th | good stain and dilution, 80 images |
| B8 | oct. 20th | BM24 15_65 1:25 without any DNA binders | oct. 20th | bad stain and to high dilution |
| B9 | oct. 20th | BM24 2DH3ML 1:25 without any DNA binders | oct. 20th | bad stain, no structures on the grid, 3 images |
| B10 | oct. 21th | BM24 15_65 1:20 without any DNA binders | oct. 21th | good stain but slightly to high dilution, 61 images |
| C1 | oct. 10th | BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp) | oct. 14th | good stain but dilution slightly to low, 32 images |
| C2 | oct. 10th | BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp) | oct. 14th | good stain but dilution slightly to low, 31 images |
| C3 | oct. 10th | BM2 1:40 without any DNA binders | ||
| C4 | oct. 10th | BM2 1:40 without any DNA binders | ||
| C5 | oct. 10th | BM2 1:40 without any DNA binders | ||
| C6 | oct. 21th | BM24 15_65 dilution 1:20 without any DNA binders | oct. 21th | stain was bad and to high dilution, 17 images |
| C7 | oct. 21th | BM24 2DH3ML dilution 1:20 without any DNA binders | oct. 21th | stain was good but dilution slightly to high, 70 images |
| C8 | oct. 21th | BM24 2DH3ML 1:20 without any DNA binders | oct. 21th | stain was good but dilution slightly to high |
| C9 | oct. 27th | BM2 1:25 without any DNA binders | oct. 27 | good stain but to high dilution, 60 images |
| C10 | oct. 27th | BM2 1:25 without any DNA binders | oct. 27th | fine grid, 60 images |
| D1 | oct. 12th | BM2 1:25 with 2.4 µM ethidium bromide (corresponding to one molecule every 7bp) | oct. 12th | good stain and dilution, 74 images |
| D2 | oct. 12th | BM2 1:25 with 0.74 µM ethidium bromide (corresponding to one molecule every 21bp) | oct. 12th | good stain and dilution, 89 images, chosen for class average |
| D3 | oct. 12th | BM2 1:25without any DNA binders | oct. 12th | good stain and dilution, 50 images |
| D4 | oct. 14th | BM2 1:25 with 432 µM DAPI (corresponding to one molecule every 7bp) | oct. 20th | good stain and structur concentration but cluster, 62 images |
| D5 | oct. 14th | BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp) | oct. 20th | stain and dilution was good, cluster, 61 images |
| D6 | oct. 27th | BM24 (15_65), 1:20 diluted with FOB20 | oct. 27th | good stain but slightly to high dilution, 22 images |
| D7 | oct. 27th | lost the grid before sample preparation | ||
| D8 | oct. 27th | BM24 (15_65) 1:10 without any DNA binders | oct. 27th | low sample concentration, 24 images |
| D9 | oct. 27th | BM24 (15_65) 1:10 without any DNA binders | stain was good but dilution slightly to low | |
| D10 | oct. 28st | BM24 (15_65) 1:5 without any DNA binders | oct. 28st | better sample concentration than D6 and D8, 60 images |
| E1 | oct. 20th | BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp) | oct. 26th | good dilution, bad staining, 49 images |
| E2 | oct. 20th | BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp) | oct. 26th | good dilution but strong background, 50 images |
| E3 | oct. 20th | BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp) | oct. 26th | fine grid, 60 images |
| E4 | oct. 20th | BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp) | oct. 26th | fine grid, 50 images |
| E5 | oct. 20th | BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp) | oct. 26th | good dilution but weak stain, 60 images |
| E6 | oct. 28st | BM24 (15_65), 1:5 | oct. 28st | fine grid, 413 images |
| E7 | oct. 31th | BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp) | oct. 31th | grid was fine, 112 images |
| E8 | oct. 31th | BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp) | oct. 31th | excellent grid, 80 images |
| E9 | nov. 1st | BM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp) | nov. 1st | good grid, 111 images |
| E10 | nov. 1st | BM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp) | nov. 1st | fine grid, 131 images |
