BioBricksAssemblyEnzymes

Assembly Standard 10 enzymes

                       Good cutter/no weird side effects

Used in the standard EcoRI Yes XbaI Yes SpeI Yes PstI Yes NotI Yes

Recommended for removal PvuII Yes XhoI Yes AvrI Don't know NheI Yes SapI Don't know

• good enzyme, ? not sure how good enzyme is, D has issues that need to be designed around if using

   * AatII
   * AscI
   ? AvrI 10(future),
   * BamHI
   * BstEII
   * EcoRI: 10,
   * EcoRV
   * HindIII
   * HpaI
   * KpnI
   * MluI
   * MscI
   * NcoI
   * NdeI
   * NheI: 10(future) 
   * NotI: 10, 
   * NsiI
   * PacI
   * PstI: 10,
   * PvuII: 10(future)
   * SacII
   * SalI
   ? SapI: 10(future)
   * ScaI
   * SfoI
   * SmaI
   * SnaBI
   * SpeI: 10,
   * SphI
   * XbaI: 10,
   * XhoI: 10(future)
   * XmnI