BME100 f2014:BME100 f2014:Group 19 L5

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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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Contents

OUR TEAM

Name: Andrew Carlson
Name: Andrew Carlson
Name: Stacy Stoddard
Name: Stacy Stoddard
Name: Gareth Palas
Name: Gareth Palas
Name: Josh Hislop
Name: Josh Hislop
Name: Josh Martin
Name: Josh Martin
Name: Jose Elenes
Name: Jose Elenes


LAB 5 WRITE-UP

Procedure

Smart Phone Camera Settings

  • Type of Smartphone:
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:


Calibration
To set up our camera in front of the fluorimeter we first had to place the lighted platform and glass slide at the back of the box with a tray under the platform to increase the height. Next, we took the phone and placed it into the phone cradle. Then we took the phone cradle and placed it in front of the glass slide, varying the distance until the drop was in focus.

  • Distance between the smart phone cradle and drop =


Solutions Used for Calibration

Initial Concentration of DNA solution(µg/mL) Volume of DNA solution(µL) Volume of SYBR Green(µL) Final DNA Solution in SYBR Green(µg/mL)
5 80 80 2.5
2 80 80 1
1 80 80 0.5
0.5 80 80 0.25
0.25 80 80 0.125
0 80 80 0



Placing Samples onto the Fluorimeter

  1. [Instructions: Step one, in your OWN words]
  2. [Instructions: Step two, in your own words]
  3. [Instructions: Step three, in your own words]
  4. [Instructions: Step etc., in your own words]


Data Analysis

Representative Images of Negative and Positive Samples


Image J Values for All Calibrator Samples


TABLE GOES HERE


Calibration curve


PCR Results Summary

  • Our positive control PCR result was ____ μg/mL
  • Our negative control PCR result was ____ μg/mL

Observed results

  • Patient _____ :
  • Patient _____ :

Conclusions

  • Patient _____ :
  • Patient _____ :




SNP Information & Primer Design

Background: About the Disease SNP


Primer Design and Testing



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