BISC220/S10: Mod 1 Media
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β-galactosidase Series Media Recipes
| Solution | Composition |
|---|---|
| Luria Broth | 1% Tryptone 0.5% Yeast Extract 1% NaCl 2% agar (only in plates) |
| Ampicillin | Stock = 25 mg/ml in water |
| IPTG (Isopropyl β-D-1-thiogalactopyranoside) | Stock 1 mg/ml |
| Na2CO3 | Stock 1M |
| Z-buffer | 60 mM Na2HPO4 60 mM NaH2PO4 1 mM MgSO4 10 mM HCl 0.27% β-mercaptoethanol |
| ONPG (ortho-Nitrophenyl-β-galactoside) | Stock 4 mg/ml |
| Laemmli Solution (2x Sample Buffer) | 0.125 M Trisma Base 4% SDS 20% glycerol 10% β-mercaptoethanol |
| Gel Running Buffer | 0.92 M Glycine 0.05 M Trisma Base 0.1% SDS |
| Coomassie Blue Stain | 0.1% Coomassie Blue 50% Methanol 10% Acetic Acid |
| Destain | 40% Methanol 7% Acetic Acid |
