BISC219/F13: Media Recipes

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Ingredients and Stock Concentrations for some of the reagents used in Series 3

Reagent Composition
Luria-Bertoni Medium (LB) 1% Tryptone
0.5% Yeast Extract
1% NaCl
(2% agar in plates)
Nematode Growth Medium Lite(NGM lite)- Available in powder form from US Biological Cat. #N1005 (http://www.usbio.net) 0.3% NaCl
1.7% Bacto-agar
0.25% Bacto-peptone
0.1% (vol/vol)cholesterol
Ampicillin* Stock = 50 mg/ml in water
use at 1:1000 in liquid cultures
use at 1:500 in LB amp plates
Tetracycline* Stock = 12.5 mg/ml in water
use at 1:1000 in liquid cultures and in solid medium plates
IPTG Stock = 0.5 M
use 0.5 mM in cultures
1x PBST (phosphate buffered saline with Tween20) 137 mM NaCl,2.7 mM KCl,4.3 mM Na2HPO4,1.47 mM KH2PO4, 0.25% -Adjust to a final pH of 7.4.
*NOTE: These concentrations are STOCK concentrations - not effective concentrations!
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