Instructors: Angela Belcher and Natalie Kuldell
TA: Eileen Higham
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.
Day 1: Screening library
Day 2: Optimizing panning
Day 3: Rescreening gold binders
Day 4: PCR of gold binding candidates
Day 5: Student presentations
Day 6: Sequence analysis
Note: PDF reprints are provided below within the context of fair use. Please obtain copies from the publisher if appropriate.
- Design criteria for engineering inorganic material-specific peptides.
Langmuir 2005 21(15):6929-6933
Peelle BR, Krauland EM, Wittrup KD, Belcher AM