BCH112106-construction of pJ23006-J23098, 99, 122, 123, 124, 125
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[j23085] + [BamHI/HindIII insert which changes key binding frame]
j23098
- PCR Extend bh005F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
- Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
- Product is pJ23006-J23098
- Screen by digesting product AlwNI/XhoI
j23099
- PCR Extend bh006F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
- Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
- Product is pJ23006-J23099
- Screen by digesting product AlwNI/XhoI
j23122
- PCR Extend bh007F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
- Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
- Product is pJ23006-J23122
- Screen by digesting product AlwNI/XhoI
j23123
- PCR Extend bh008F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
- Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
- Product is pJ23006-J23123
- Screen by digesting product AlwNI/XhoI
j23124
- PCR Extend bh009F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
- Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
- Product is pJ23006-J23124
- Screen by digesting product AlwNI/XhoI
j23125
- PCR Extend bh010F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
- Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
- Product is pJ23006-J23125
- Screen by digesting product AlwNI/XhoI