26 February 2009

Date: 26 February 2009; Time:1400; Agenda: Fixing biofilm and airdrying for SEM.

• The lids containing biofilm that were exposed to the polymers overnight were removed from the challenge plates and briefly rinsed in PBS solution for 2 minutes each to remove any planktonic bacteria.

• The bioflim was then fixed in a fixing solution:

Preparation of fixing solution:

• 12ml of 25% glutaldehyde was mixed with 100ml 0.1M PBS.
  • To prepare 0.1M PBS:
  • 20 tablets of PBS were dissolved in 400ml of water

• This fixing solution was then added to 2 new CBD plates and the lids containing biofilm replaced. They were left to soak in the fixing solution for 2 hrs.

• After 2 hrs, the lids were removed and put into a styrofoam box (with holes above) to air dry in the fume hood for 5 days (ie. Till next Tues).