10X BPTE electrophoresis buffer

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Contents

Purpose

Used as the electrophoresis buffer during denaturing (via glyoxal) agarose gel electrophoresis of RNA.

Components

  • 100 mM PIPES
  • 300 mM Bis-Tris
  • 10 mM EDTA

The final pH of this 10x buffer is ~6.5.

Procedure

  1. Prepare by adding the following to 90 ml of distilled H2O
    • 3 g of PIPES (free acid)
    • 6 g of Bis-Tris (free base)
    • 2 ml of 0.5 M EDTA
  2. Treat the solution with final concentration of 0.1% DEPC for 1 hour at 37°C
  3. Autoclave.

References

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