-80 Glycerol Stocks

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Protocol

  • note: only make ONE -80 tube for each sample
  • mix 150μL of your freshly grown culture with 150μL of 50% glycerol in a barcode tube
  • scan each tube on Simple Scan 1000 (on the computer next to the robot)
    • enter data, and click save
  • log position and data into [hhttps://spreadsheets.google.com/ccc?key=0AvjDNAea5YizdENMN05Wc3R0LTRUX3djUmJ3ZS1rV2c&hl=en#gid=13 google docs file]
  • store in -80
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