基礎ゼミチーム/basic seminar team/project

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BIOMOD2012 Tohoku Team B
Our Robot, "D-NApper"
D-NApper can catch and store the target you like.
So, what DNA structure do we need to build D-NApper?

-We have three ideas.
  1. capture the target and move it close to D-NApper, even if there are few targets.
  2. put the target inside D-NApper.
  3. select the target with specificity.
Body Structure
We designed the body structure like a hexagonal tube. Because we think the body structure has to be long enough to put things inside, and the hexagonal tube is relatively easy to design. The body structure is 22 nm across and 34 nm long. We adopted a double layer of honeycomb lattice to ensure the strength.
Selector
To realize these three ideas, we propose Selector structure. Selector is a long single-stranded DNA which has complementary sequences to the target. Between this complementary sequences are adenine sequences which are expected to act as a loop.

We have three Selectors. The inner Selector attaches to the target more strongly. When Selector1 hybridizes with the target sequence, the Selector 1 shrinks, making two single loops between the hybridized parts. Thanks to the Selector1, D-Napper can select and move the target close. Next, as the inner Selector attaches to the target more strongly, the target moves inside. Selector 2 receives the target from Selector1. And then Selector3 receives the target from Selector2. Thanks to Selector2 and Selector3, D-NApper is likely to deliver the target inside.

So the three Selectors are designed to capture the target, put into D-NApper, and also sort the target. Three selectors are on one inner surface of the hexagonal tube.

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