Yu:NP40 Buffer: Difference between revisions
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Michaelcyu (talk | contribs) (NP40 Buffer) |
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Revision as of 11:45, 14 June 2009
| 1. Measure 21mL 0.5M Na2HPO4 (sodium phosphate dibasic) into a 1L beaker for a final concentration of 15mM Na2HPO4 | 21mL 0.5M Na2HPO4 |
| 2. Add 14mL 0.5M NaH2PO4 (sodium phosphate monobasic) for a final concentration of 10mM NaH2PO4 | 14mL 0.5M NaH2PO4 |
| 3. Add 70mL 10% NP-40 for a final concentration of 1% NP-40 | 70mL 10% NP-40 |
| 4. Add 21mL 5M NaCl for a final concentration of 150mM NaCl | 21mL 5M NaCl |
| 5. Add 2.8mL 0.5M EDTA for a final concentration of 2mM EDTA | 2.8mL 0.5M EDTA |
| 6. Add 2.31g NaF for a final concentration of 50mM NaF | 2.31g NaF |
| 7. Add 0.7mL Na3VO4 for a final concentration of 0.1mM Na3VO4 | 0.7mL Na3VO4 |
| 8. Bring volume to 700mL total with millipore H2O in a graduated cylinder | Bring volume to 700mL |
