User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/16: Difference between revisions
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[[Image:HRP and Citrate AuNP.png]] | [[Image:HRP and Citrate AuNP.png]] | ||
Making the BSA AuNP solution | |||
Add 1mL of the 2.54mM gold (HAuCl4) solution to a 10mL volumetric flask | |||
Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA | |||
- Calculations = ((0.00254 moles/L Gold)* (1L/1000ml)* (1ml)) / 90 = moles BSA = 2.82*10^-8 moles BSA | |||
[BSA] = 15.6μM | |||
- (2.82*10^-8 moles) * (1L/15.6*10^-6 moles) = 0.00180L or 1.8mL BSA to add to solution | |||
Add deionized water up to 10mL | |||
Transfer solution to a test tube and cap with aluminum foil | |||
Heat in oven at 80C for 3 hours | |||
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Revision as of 11:50, 16 October 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveWe are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol Description
DataStock Solution
Notes- We measured the absorbance of the 1/20 luminol to see if it still absorbed. We found the absorbance peak to be less than 1.00, specifically around 0.300 Graph showing the absorbance spectra for citrate AuNP and HRP AuNP Making the BSA AuNP solution Add 1mL of the 2.54mM gold (HAuCl4) solution to a 10mL volumetric flask Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA - Calculations = ((0.00254 moles/L Gold)* (1L/1000ml)* (1ml)) / 90 = moles BSA = 2.82*10^-8 moles BSA [BSA] = 15.6μM - (2.82*10^-8 moles) * (1L/15.6*10^-6 moles) = 0.00180L or 1.8mL BSA to add to solution Add deionized water up to 10mL Transfer solution to a test tube and cap with aluminum foil Heat in oven at 80C for 3 hours |