User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/16
From OpenWetWare
Project name | Main project page Previous entry Next entry |
ObjectiveWe are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol Description
DataStock Solution
NotesFigure 1: Absorbance spectra of the catalytic activity of HRP-AuNPs
- We measured the absorbance of the 1/20 luminol to see if it still absorbed. We found the absorbance peak to be less than 1.00, specifically around 0.300 Figure 2: Graph showing the absorbance spectra for citrate AuNP and HRP AuNP Making the BSA AuNP solution -Add 1mL of the 2.54mM gold (HAuCl4) solution to a 10mL volumetric flask -Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA - Calculations = ((0.00254 moles/L Gold)* (1L/1000ml)* (1ml)) / 90 = moles BSA = 2.82*10^-8 moles BSA [BSA] = 15.6μM - (2.82*10^-8 moles) * (1L/15.6*10^-6 moles) = 0.00180L or 1.8mL BSA to add to solution Add deionized water up to 10mL Transfer solution to a test tube and cap with aluminum foil Heat in oven at 80C for 3 hours |