User:Wesley J. Houston/Notebook/MAP/2013/03/21: Difference between revisions
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==3/21/13== | ==3/21/13== | ||
* Retrieved liquid cultures from the incubator. | * Retrieved liquid cultures from the incubator. | ||
* Pelleted the samples (3 mins @ 16.3k | * Pelleted the samples (3 mins @ 16.3k x g). | ||
** No pellets of KAH201 were produced. | ** No pellets of KAH201 were produced. | ||
** KAH182 was successfully pelleted. | ** KAH182 was successfully pelleted. | ||
* Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182. | * Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182. | ||
** Concentration and purity was also determined | ** Concentration and purity was also determined. | ||
*** Note there were two samples of KAH182. | *** Note there were two samples of KAH182. | ||
* Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies. | * Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies. | ||
** Following the established protocol for bacterial transformation. | ** Following the established protocol for bacterial transformation. | ||
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table --> | |||
|- | |||
| <u>Sample</u> || <u>260/280</u> || <u>ng/μL</u> | |||
|- | |||
| 1. KAH182 (Plasmid) || 1.758 || 43.201 | |||
|- | |||
| 2. KAH182 (Plasmid) || 7.751 || 40.434 | |||
|} | |||
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Latest revision as of 22:34, 26 September 2017
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3/21/13
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