User:Wesley J. Houston/Notebook/MAP/2013/03/22

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3/22/13 Gel purification was performed using the KAH201 plasmids prepped on 2/28, as well as the KAH182 plasmids prepped on 3/21. No DNA was present in the second lane, and the concentrations and purities were determined to be too low to complete a ligation. Grew more KAH201 liquid cultures. Placed colonies in LB broth overnight in shaking 37C incubator. Reagent Volume   30 μL/lane, 1% agarose; Ladder DNA (plasmid) 20 μL 10x buffer 3.0 enzyme 1 (Pst1) 1.0 enzyme 2 (Xba1 or Spe1) 1.0 dH2O 5.0 μL   30 μL --> 37°C/ ~15 min. > Measure conc.'s of gel purified fragments Sample 260/280 ng/μL 1. KAH201 (S/P) 0.32 0.851 2. KAH182 (X/P) 0.385 1.064