User:Torsten Waldminghaus/Primer
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- All primers are in concentrations of 100pmol/μL
Name | Sequence | Characteristics | Primer number |
---|---|---|---|
GFPrv | ctgatttaatctgtatcaggc | The following primers are for the mutagenesis of GFP to introduce GATC sites as silent mutation (GFPrv, GFPfw, GFPmut1fw, GFPmut2rv, GFPmut3fw, GFPmut4rv, GFPmut5fw, GFPmut6fw, GFPmut7fw, GFPmut8fw, GFPmut9fw) Tm 55 (salt adjusted)[1] | 1 |
GFPfw | CTCTCTACTGTTTCTCCATAC | Tm 57 | 2 |
GFPmut1fw | caaacaaaagaatggGatcaaagctaac | Tm 62 [without mutated nucleotide] | 3 |
GFPmut2rv | caatgttgtggcgGatCttgaagttag | Tm 63 [without mutated nucleotide] | 4 |
GFPmut3fw | caactagcagaTcattatcaacaaaatac | Tm 63 [without mutated nucleotide] | 5 |
GFPmut4rv | gccatcgccGatCggagtattttg | Tm 62 [without mutated nucleotide] | 6 |
GFPmut5fw | cgaaaagcgtgaTcacatggtcc | Tm 64 [without mutated nucleotide] | 7 |
GFPmut6fw | ctgctgctgggatCacacatggc | Tm 66 [without mutated nucleotide] | 8 |
GFPmut7fw | GTTATCCGGACCATATGAAACGGC | 5’-phosph. HPLC | 9 |
GFPmut8fw | CATTGAAGATGGGTCCGTTCAACTAG | 5’-phosph. HPLC | 10 |
GFPmut9fw | CCTTTCGAAAGACCCCAACGAAAAG | 5’-phosph. HPLC | 11 |
GATC19DNAfw | GCCCGCGGATCCGCCCGCC | oligo for methylation experiment from A. Humeny et al. 2003 | 12 |
GATC19DNArv | GGCGGGCGGATCCGCGGGC | oligo for methylation experiment from A. Humeny et al. 2003 | 13 |
pyrBfw | ggatcCACCCATTCCCAGCCCCTC | cloning of pyrB | 14 |
pyrBrv | gaattcCCTTACAGTACCAGATCGCG | cloning of pyrB | 15 |
MseIlong | AGTGGGATTCCGCATGCTAGT | 16 | |
MseIshort | TAACTAGCATCG | This sequence seems to be wrong because the last CG is not complementary to MseIlong but should instead be GC => use MseIshortnew instead | 17 |
MseIshortnewNo | TAACTAGCATGC | Not phosphorilated | 18 |
MseIshortnew | TAACTAGCATGC | 5'modified with phosphate | 19 |
bet-fw | GTCGACCCACAGGAACTGAT | To distinguish DY330 (with lambda phage) from other E. coli strains use primers for bet as part of the recombination machinery of lambda | 20 |
bet-rev | GGCTGACGTTCTGCAGTGTA | To distinguish DY330 (with lambda phage) from other E. coli strains use primers for bet as part of the recombination machinery of lambda | 21 |
Cut-test_fw | TAATAGGCATGCTAGCAGCTG AGCGTAGCTAGCGATGCAACGAC GGTGATCAGCGTCGATGCAGCCG AAACGATGACTGTCACATAGCTG ATGCTTTGCT | ||
Cut_test_rv | TAAGCAAAGCATCAGCTATGT GACAGTCATCGTTTCGGCTGCAT CGACGAAGATCACCGTCGTTGCA TCGCTAGCTACGCTCAGCTGCTA GCATGCCTAT | ||
Cut-test_fw | TAATAGGCATGCTAGCAGCTG AGCGTAGCTAGCGATGCAACGAC GGTGATCAGCGTCGATGCAGCCG AAACGATGACTGTCACATAGCTG ATGCTTTGCT | ||
Cut_test_rv | TAAGCAAAGCATCAGCTATGT GACAGTCATCGTTTCGGCTGCAT CGACGAAGATCACCGTCGTTGCA TCGCTAGCTACGCTCAGCTGCTA GCATGCCTAT | ||
Cm_seqF | |||
Cm_seqR | |||