User:Tara K. Luckau/Notebook/Team ConGen/2013/05/15

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(Autocreate 2013/05/15 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
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==CNTI Acos5==
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==SCOC MP2==
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Last week's didn't work, presumably because the DNAs were plated a couple months ago. Re-plated Plate 5. Expect it's fine, then will go ahead and re-plate the rest of SCOC MP2 and PCR.
===PCR===
===PCR===
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* [[Image:20130110_PCRa.png|800 px]]
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* [[Image:20130515_PCR.png|800 px]]
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===Gel===
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{| {{table}} style="text-align: center"
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|-
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! Pour !! Load !! Run
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|-
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
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|-
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| 27 µL GelRed || 6 µL ladder || 45 minutes
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|}
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[[Image:20130110_Gela.png|800 px]]
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* mispriming everywhere - primer pair not usable for CNHY
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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:: [[Image:20130227_Frag.png|900 px]]
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:: [[Image:20130515_Frag.png|900 px]]
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151779 801124151779]
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124713155 801124713155]
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:: [[Image:20130227_FedEx.png|700 px]]
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:: [[Image:20130515_FedEx.png|700 px]]
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* UAGC Submission# ????
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* UAGC Submission# PZZN
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* FedEx picked up ____pm, 26 February
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* FedEx picked up ____pm, 15 May
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* FedEx delivered ____am, 27 February
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* FedEx delivered ____am, 16 May
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* UAGC received ____am, 27 February
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* UAGC received ____am, 16 May
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* UAGC completed ____am, 28 February
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* UAGC completed ____am, 17 May

Revision as of 17:27, 15 May 2013

Tara's Lab Notebook Main project page
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Contents


SCOC MP2

Last week's didn't work, presumably because the DNAs were plated a couple months ago. Re-plated Plate 5. Expect it's fine, then will go ahead and re-plate the rest of SCOC MP2 and PCR.


PCR


Fragment Analysis Submission



  • UAGC Submission# PZZN


  • FedEx picked up ____pm, 15 May
  • FedEx delivered ____am, 16 May
  • UAGC received ____am, 16 May
  • UAGC completed ____am, 17 May




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