User:Tara K. Luckau/Notebook/Team ConGen/2013/05/15: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/05/15 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
No edit summary
Line 9: Line 9:




==CNTI Acos5==
==SCOC MP2==
Last week's didn't work, presumably because the DNAs were plated a couple months ago. Re-plated Plate 5. Expect it's fine, then will go ahead and re-plate the rest of SCOC MP2 and PCR.




===PCR===
===PCR===
* [[Image:20130110_PCRa.png|800 px]]
* [[Image:20130515_PCR.png|800 px]]
 
 
===Gel===
 
{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
 
 
[[Image:20130110_Gela.png|800 px]]
 
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




Line 38: Line 20:




:: [[Image:20130227_Frag.png|900 px]]
:: [[Image:20130515_Frag.png|900 px]]




* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151779 801124151779]
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124713155 801124713155]
:: [[Image:20130227_FedEx.png|700 px]]
:: [[Image:20130515_FedEx.png|700 px]]




* UAGC Submission# ????
* UAGC Submission# PZZN




* FedEx picked up ____pm, 26 February
* FedEx picked up ____pm, 15 May
* FedEx delivered ____am, 27 February
* FedEx delivered ____am, 16 May
* UAGC received ____am, 27 February
* UAGC received ____am, 16 May
* UAGC completed ____am, 28 February
* UAGC completed ____am, 17 May




Revision as of 14:27, 15 May 2013

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


SCOC MP2

Last week's didn't work, presumably because the DNAs were plated a couple months ago. Re-plated Plate 5. Expect it's fine, then will go ahead and re-plate the rest of SCOC MP2 and PCR.


PCR


Fragment Analysis Submission



  • UAGC Submission# PZZN


  • FedEx picked up ____pm, 15 May
  • FedEx delivered ____am, 16 May
  • UAGC received ____am, 16 May
  • UAGC completed ____am, 17 May




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Tara_K._Luckau/Notebook/Team_ConGen/2013/05/15&oldid=698138"