Objective
- To perform FTIR, DSC and XRD analysis on AuNP lysozyme fibers.
Description
The protocol for today can be found here.
- Analysis Prep
- 10 5mL Fiber samples were transferred into 5 15mL centrifuge tubes.
- Samples were then centrifuged for 5 mins at 4000 R.
- XRD
- Droplets of fibers were placed on a small amount of PlayDoh and kneaded together. The combination pf PlayDoh and fibers was placed into a lid specific for XRD.
- DSC
- Sample 1: AuNP Fiber
- Pan & Lid: 51.37 mg
- Sample: 3.49 mg
- Together: 53.73 mg
- Sample 2: AuNP Fiber
- Pan & Lid: 50.80 mg
- Sample: 4.43 mg
- Together: 53.74 mg
- Sample 3: AuNP Fiber
- Pan & Lid: 50.71 mg
- Sample: 3.63 mg
- Together: 54.44 mg
- Sample 4: Lysozyme
- Pan & Lid: 51.237 mg
- Sample: 3.04 mg
- Together: 54.38 mg
- Sample 1 was placed in well 27 in the DSC apparatus. Samples 2, 3 and 4 were placed in wells 28, 29 and 30, respectively.
- Cycling Settings:
- Equilibrate at 20°C
- Ramp up to 5°C/min and to 100°C.
- Isothermal - 3 mins
- Ramp down to 5°C/min and to 20°C
- Repeat (blue arrow)
- Lastly, external event (OFF) <= (only for the last sample was this configuration added.)
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