User:Sydney Marshall/Notebook/Protease Project/2015/09/23

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Objective

  • To perform FTIR, DSC and XRD analysis on AuNP lysozyme fibers.


Description

The protocol for today can be found here.

  • Analysis Prep
    • 10 5mL Fiber samples were transferred into 5 15mL centrifuge tubes.
    • Samples were then centrifuged for 5 mins at 4000 R.
  • XRD
    • Droplets of fibers were placed on a small amount of PlayDoh and kneaded together. The combination pf PlayDoh and fibers was placed into a lid specific for XRD.
  • DSC
    • Sample 1: AuNP Fiber
      • Pan & Lid: 51.37 mg
      • Sample: 3.49 mg
      • Together: 53.73 mg
    • Sample 2: AuNP Fiber
      • Pan & Lid: 50.80 mg
      • Sample: 4.43 mg
      • Together: 53.74 mg
    • Sample 3: AuNP Fiber
      • Pan & Lid: 50.71 mg
      • Sample: 3.63 mg
      • Together: 54.44 mg
    • Sample 4: Lysozyme
      • Pan & Lid: 51.237 mg
      • Sample: 3.04 mg
      • Together: 54.38 mg
  • Sample 1 was placed in well 27 in the DSC apparatus. Samples 2, 3 and 4 were placed in wells 28, 29 and 30, respectively.
  • Cycling Settings:
    • Equilibrate at 20°C
    • Ramp up to 5°C/min and to 100°C.
    • Isothermal - 3 mins
    • Ramp down to 5°C/min and to 20°C
    • Repeat (blue arrow)
    • Lastly, external event (OFF) <= (only for the last sample was this configuration added.)



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