User:Sean E. Lauber: Difference between revisions
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*[[OpenWetWare:Welcome|Introductory tutorial]] | *[[OpenWetWare:Welcome|Introductory tutorial]] | ||
*[[Help|OpenWetWare help pages]] | *[[Help|OpenWetWare help pages]] | ||
====Technical guides==== | |||
*[[http://www.dako.com/08002_ihc_staining_methods_5ed.pdf|Dako's IHC Guide]] | |||
Revision as of 13:56, 31 October 2012
Contact Info
- sean.laub@gmail.com
Education
- M.Sc Candidate, Medical Sciences (Immunology), McMaster University, Hamilton, ON 2010-2012
- B.Sc, Honors Cell & Molecular Biology, Concordia University, Montreal, QC 2005-2010
- DEC, Natural Sciences, John Abbott College, Montreal, QC 2002-2004
Protocols
Richards Lab Protocols
Molecular Techniques
- Cytokeratin IHC staining
- ELISA Development
- Isotype ELISA against Adenovirus
- Generating Adenovirus Lysate
- PCR
- Stable Transfection Using Lipofectamine 2000
- RNA Extraction Using Trizol
- DNA Extraction Using Trizol
- DNase Treatment Using DNA-Free (for Taqman)
- Reverse Transcription using Superscript II (for Taqman)
- qRT-PCR (TaqMan)
Mammalian Cell Culture
- Freezing Mammalian Cells
- Thawing Mammalian Cells
- Culturing Lewis Lung Carcinoma Cells
- MTT Cell Proliferation Assay
- Cytokine Stimulation of Cells
- Culturing of Primary Macrophages
Technical
- Agilent Bioanalyzer
- Statistics in Graphpad
- AMIRA - Creating Label Fields by Lung Segmentation
- AMIRA - Generating Histograms
- AMIRA - Density Overlays
- ImageJ - Threshold Analysis
- Using the 7900 HT qPCR Machine
- Heat maps in Excel
Animal Work
- Broncho-alveolar Lavage
- Peritoneal Lavage
- Formalin Fixation & Preparation for Histology
- Mouse Endotracheal Intubation
- Using the Kras G12D Mouse Model for Lung Cancer
- Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- Kras G12D Genotyping
- Differential cell staining
Joyce Lab Protocols
- DNA Electrophoresis
- Bacterial Transformation
- T-tailing pBSKS
- Ethanol precipitation of DNA
- DNA Phenol extraction
- Preparing RNA from yeast
- Extraction of DNA from an agarose gel
- DNA Ligation
- PCR Mutagenesis
Links
Protocols and general information
- Recipes and protocols for stock solutions, competent cells, mini/midi/maxi prep and more
- Protocol-online Protocols
- Biowww.net Protocols
- Restriction digests
- Restriction digest troubleshooting
- More troubleshooting
- Journal of Visualized Experiments
- PSORT
Useful links
- Bitesize Bio
- BioForum
- Scientist Solutions
- Biochemweb.org
- E. coli Genotypes
- Online Mendelian Inheritance in Man
- NCBI
- Uniprot (as good as NCBI)
- Nebcutter 2.0
- Translate DNA
- Promoter Predictor
- Download the Molecular Biology Toolbar
- Backup your data with Mozy
