Joyce: Extraction of DNA from an agarose gel

1. Remove bands from gel on the preparative setting of a UV illuminator
2. Place in an ependorff
3. Mash up bands
4. Add an equal volume of phenol
5. Vortex for 2 minutes
6. Place at -70°C for 30 minutes
7. Thaw for 10 minutes at 37°C
8. Repeat steps 4-7
9. Spin at max speed for 1 minute
10. Add 20 ul of 3M NaOAc (pH 5.2)
11. Spin at full speed for 5 minutes
12. Pipette off aqueous phase into a new ependorff
13. Adjust salt concentration to 0.3 M
14. Extract with 1x phenol, 2x ether, and precipitate