User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/08/31: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
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==Objective== | ==Objective== | ||
Observe a change in concentration of gold nanoparticles in solutions which vary in pH and Fructose concentration. The change in gold nanoparticle concentration can be seen from a change of relevant light absorbance, measured with a spectrophotometer | |||
==Description== | ==Description== | ||
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| 8 || 0 || 5 || 0 || 374 || 24 || 460 || 4137 || increased || purple | | 8 || 0 || 5 || 0 || 374 || 24 || 460 || 4137 || increased || purple | ||
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| 9 || 0 || 50 || 0 || 374 || 24 || 460 || 4092 || | | 9 || 0 || 50 || 0 || 374 || 24 || 460 || 4092 || no change || purple | ||
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| 10 || 0 || 500 || 0 || 374 || 24 || 460 || 3642 || decreased || purple | | 10 || 0 || 500 || 0 || 374 || 24 || 460 || 3642 || decreased || purple | ||
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pH 7 is not applicable because the gold solution had produced fibers. We discarded it and did not collect data with it. | |||
Solutions were prepared by [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/08/31 | Dr Hartings]] | |||
==Data analysis== | ==Data analysis== | ||
Gold nanoparticles are of purple color. The spectrophotometer can therefore detect the presence of gold nanoparticles when it measures absorbance of light in the UV and visible wavelengths. Gold nanoparticles absorb light with a wavelength of around 530 nm. | |||
The general trend, independent of Fructose presence, shows that absorbance at the relevant wavelength (around 530 nm) increases slightly with increasing pH, which means the concentration of nano particles increased slightly. | The general trend, independent of Fructose presence, shows that absorbance at the relevant wavelength (around 530 nm) increases slightly with increasing pH, which means the concentration of nano particles increased slightly. | ||
For high pH values, starting at pH = 11, there seem to be no gold nano particles at all. The solutions are transparent, and show no peak in absorption. | For high pH values, starting at pH = 11, there seem to be no gold nano particles at all. The solutions are transparent, and show no peak in absorption. | ||
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[[Image:UV Vis 08.31 SB graph2.png | Figure 2]] | [[Image:UV Vis 08.31 SB graph2.png | Figure 2]] | ||
[[Image:Uv gold 0.0625 NC.PNG | Figure 7]] | |||
[[Image:Uv gold 0.0625mM BC.PNG| Figure 8]] | |||
[[Image:A530 gold 0mM NC.PNG | Figure 9]] | |||
[[Image:SB08.31.UVVispng | Figure 3]] | [[Image:SB08.31.UVVispng | Figure 3]] | ||
[[Image:SB08.31.UV Vis Graph 5.png | Figure 6]] | |||
[[Image:SB08.31.UVVIS.png | Figure 4]] | [[Image:SB08.31.UVVIS.png | Figure 4]] | ||
==Notes== | ==Notes== |
Latest revision as of 01:53, 27 September 2017
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UV VisObjectiveObserve a change in concentration of gold nanoparticles in solutions which vary in pH and Fructose concentration. The change in gold nanoparticle concentration can be seen from a change of relevant light absorbance, measured with a spectrophotometer DescriptionCompare absorption of UV-Vis of solutions containing 0.25 mM Gold with
Composition of solutions:
pH 7 is not applicable because the gold solution had produced fibers. We discarded it and did not collect data with it. Solutions were prepared by Dr Hartings Data analysisGold nanoparticles are of purple color. The spectrophotometer can therefore detect the presence of gold nanoparticles when it measures absorbance of light in the UV and visible wavelengths. Gold nanoparticles absorb light with a wavelength of around 530 nm. The general trend, independent of Fructose presence, shows that absorbance at the relevant wavelength (around 530 nm) increases slightly with increasing pH, which means the concentration of nano particles increased slightly. For high pH values, starting at pH = 11, there seem to be no gold nano particles at all. The solutions are transparent, and show no peak in absorption. The difference between 0 mM Fructose and 0.0625 mM Fructose seems to be that latter has higher absorbance for pH 4 and pH 9. For all other pH values, the Fructose does not seem to affect the Au nano concentration significantly. One thing to be careful with when analyzing the peak values is to take into account potential shifts in the base line. The graphs below show both original results, and baseline-corrected results of absorption. The highest concentration of gold nano particles was for 0.0625 and pH 10. NotesCuvette can be cleaned with DI water, 10 percent HCl (because we are using Gold) and soap. Water has to come last. It should be dried with blowing air. Be cautious with air, can shoot off glass pipette at tip of tube if air comes out too strong. for SILVER use nitric acid for cleaning !
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