User:Sara K. Mattson/Notebook/Chemistry 671/2016/11/30: Difference between revisions
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==Procedure== | ==Procedure== | ||
First pH 11 was made: | |||
#Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total | #Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total | ||
##Au 3.37mM | ##Au 3.37mM | ||
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##Scans were taken at 80oC every 2 minutes for 3 hours | ##Scans were taken at 80oC every 2 minutes for 3 hours | ||
Second pH 12 was made: | |||
#Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total | |||
##Au 3.37mM | |||
###(0.25mM)(3.0mL)=(XmL)(3.37mM) | |||
###XmL= .223mL= 223uL Au | |||
##Myoglobin 230uM | |||
###(12.5uM)(3.0mL)=(XmL)(230uM) | |||
###X=0.163mL= 163uL Myoglobin | |||
##30uL 1M NaOH | |||
##Water | |||
###Volume of water= 3000uL- 223uL-163uL-30uL= 2584uL | |||
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Revision as of 12:10, 30 November 2016
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Ocean OpticsPurposeTo use ocean optics to make conclusions about the rate of myoglobin templated Au nanoparticle formation in pH 11 and 12 ProcedureFirst pH 11 was made:
Second pH 12 was made:
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