User:Sara K. Mattson/Notebook/Chemistry 671/2016/12/06

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Fluorescence Purpose Use fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH 11 and pH 12 Procedure pH 11 Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total Au 3.37mM (0.25mM)(3.0mL)=(XmL)(3.37mM) XmL= .223mL= 223uL Au Myoglobin 230uM (12.5uM)(3.0mL)=(XmL)(230uM) X=0.163mL= 163uL Myoglobin 3uL 1mM NaOH Water Volume of water= 3000uL- 223uL-163uL-3uL= 2611uL Absorbance measurements were taken and saved every 3 minutes for 3 hours The instrument was set at 80 degrees Celsius The scan range parameters were start at 310nm and ending at 540nm The emission and exit slit were set at 10nm pH 12 Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total Au 3.37mM (0.25mM)(3.0mL)=(XmL)(3.37mM) XmL= .223mL= 223uL Au Myoglobin 230uM (12.5uM)(3.0mL)=(XmL)(230uM) X=0.163mL= 163uL Myoglobin 30uL 1mM NaOH Water Volume of water= 3000uL- 223uL-163uL-30uL= 2584uL Absorbance measurements were taken and saved every 3 minutes for 3 hours The instrument was set at 80 degrees Celsius The scan range parameters were start at 310nm and ending at 540nm The emission and exit slit were set at 10nm Data Data for pH 11 pH 11 Scans pH 11 Wavelength pH 11 Integrated Intensity Data for pH 12 pH 12 Scans pH 12 Wavelength pH 12 Integrated Intensity