User:Samiye Yaman/Notebook/Lab 581/2013/03/27: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Description==
==Description==
'''Part 1: Freeze-thaw vortex cycle of liposomes'''
 
*5 freeze-thaw vortex cycle for liposome coated hydrogel. (vortex for 30sec, freeze (completely) by using liquid nitrogen then thaw (completely)in a water bath. Repeat the cycle 5 times.
 
*Centrifuge the solution(4000rpm for 5 min) then remove the 25mM Tris solution from the epi-test tube.
 
*Add 1.5 mL of 25mM Tris buffer back to the epi test tube with hydrogels.
 
 
'''Part 2: Prepare a concentration range for R6G'''
 
*Add 2mg of PVOH to 6 different epi test tubes.
 
*Add 1.5 ml of phosphate buffer.
 
*Add different volume of 1mM R6G to each epi test tube. (10μL, 15μL, 20μL, 25μL, 30μL, 35μL)
 
 
'''Part 3: Preparing hydrogels for pH analysis'''
 
* Centrifuge hydrogel solutions with containing methyl red and bromophenol blue prepared on 3/22
 
* After the centrifuge, collect supernatant and place it in to a sepetrate epi-tube.
 
*Add 1mL phosphate buffer to hyrdogels.(repeat the process 3 times.
 
*Uv-Vis of measurement of each supernantant was done.Additionally stock solution of the dyes were also measured with UV-vis.
 
==Data==
==Data==


==Notes==
==Notes==

Latest revision as of 22:34, 26 September 2017

Experimental Chemistry Main project page
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Objective

Description

Part 1: Freeze-thaw vortex cycle of liposomes

  • 5 freeze-thaw vortex cycle for liposome coated hydrogel. (vortex for 30sec, freeze (completely) by using liquid nitrogen then thaw (completely)in a water bath. Repeat the cycle 5 times.
  • Centrifuge the solution(4000rpm for 5 min) then remove the 25mM Tris solution from the epi-test tube.
  • Add 1.5 mL of 25mM Tris buffer back to the epi test tube with hydrogels.


Part 2: Prepare a concentration range for R6G

  • Add 2mg of PVOH to 6 different epi test tubes.
  • Add 1.5 ml of phosphate buffer.
  • Add different volume of 1mM R6G to each epi test tube. (10μL, 15μL, 20μL, 25μL, 30μL, 35μL)


Part 3: Preparing hydrogels for pH analysis

  • Centrifuge hydrogel solutions with containing methyl red and bromophenol blue prepared on 3/22
  • After the centrifuge, collect supernatant and place it in to a sepetrate epi-tube.
  • Add 1mL phosphate buffer to hyrdogels.(repeat the process 3 times.
  • Uv-Vis of measurement of each supernantant was done.Additionally stock solution of the dyes were also measured with UV-vis.

Data

Notes
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