User:Robwarden/Notebook/Caspase-3 Activatable Probe/2009/09/11: Difference between revisions
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==Transformation== | ==Transformation== | ||
* | *Stbl2 Cells | ||
*Shook at 30°C for 1:30. | *Shook at 30°C for 1:30. | ||
*Plated on LB-Amp. | *Plated on LB-Amp. | ||
*Incubated overnight at | *Incubated overnight at 30°C | ||
Revision as of 12:10, 11 September 2009
Caspase-3 Activatable Probe | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
PCR ValidationAgarose Gel Purification
Digestion5.0 μL 10X Buffer EcoRI 0.5 μL 100X BSA 30.0 μL PCR Product / 1 μg Vector (1X and 5X) 1.0 μL EcoRI 1.0 μL XhoI 12.5 μL dH2O / to 50 μL
Reaction Cleanupusing Qiagen PCR Cleanup Kit Ligation2.0 μL 10X T4 Ligase Buffer 2.5 μL Vector Backbone 7.5 μL hgLuc Insert 7.0 μL MQ 1.0 μL T4 DNA Ligase
Transformation
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