User:Robwarden/Notebook/Caspase-3 Activatable Probe/2009/09/10
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with Qiagen kit
Colonies 2, 5, 7, and 13 were submitted for sequencing. T7 was used for the forward primer. BHG was used for the reverse primer. Both primers were supplied by the sequencing facility.
PCR of hRLuc8
5.0 μL 10X Buffer 1.0 μL hRLuc8 plasmid 2.5 μL Primer "hRLuc8_EcoRI_Fwd" 2.5 μL Primer "hRLuc8_XhoI_Rev" 1.0 μL 10mM dNTP mix 0.5 μL Vent Polymerase 37.5 μL dH2O