User:Puja Mody/Notebook/Magnetite Shenanigans/2014/09/22: Difference between revisions
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* Standards needed to be made of R6G in order to calculate the amount of dye that was absorbed and how much dye remains in the solution. | * Standards needed to be made of R6G in order to calculate the amount of dye that was absorbed and how much dye remains in the solution. | ||
Molar Absorptivity of R6G: 116000 LMol<sup>-1</sup>cm<sup>-1</sup> | |||
Assumed stock concentration: .005 M | Assumed stock concentration: .005 M | ||
*Because the absorbance of R6G is so high, standard concentrations needed to be diluted to below 8 μM. | |||
Table 1. Standard concentrations in 2mL solutions | Table 1. Standard concentrations in 2mL solutions | ||
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*The concentration of the original stock solution was 398.7μM | |||
Table 2: Actual concentration of dye in eppendorf samples | Table 2: Actual concentration of dye in eppendorf samples | ||
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==Notes== | |||
-These samples were left for too long without being tested and resulted in some of the dye releasing out of the complexes. For this reason, UV-Vis of these samples were not fully run and new samples were made. | -These samples were left for too long without being tested and resulted in some of the dye releasing out of the complexes. For this reason, UV-Vis of these samples were not fully run and new samples were made. | ||
Revision as of 15:42, 23 September 2014
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UV-Vis standards
Molar Absorptivity of R6G: 116000 LMol-1cm-1 Assumed stock concentration: .005 M
Table 1. Standard concentrations in 2mL solutions
Table 2: Actual concentration of dye in eppendorf samples
Notes-These samples were left for too long without being tested and resulted in some of the dye releasing out of the complexes. For this reason, UV-Vis of these samples were not fully run and new samples were made.
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