User:Puja Mody/Notebook/Magnetite Shenanigans/2014/09/22

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UV-Vis standards

  • Standards needed to be made of R6G in order to calculate the amount of dye that was absorbed and how much dye remains in the solution.

Molar Absorptivity of R6G: 116000 LMol-1cm-1 Assumed stock concentration: .005 M

  • Because the absorbance of R6G is so high, standard concentrations needed to be diluted to below 8 μM.

Table 1. Standard concentrations in 2mL solutions

Standard Concentrations (uM) Actual Concentration (M) Absorbance
000
21.59483E-060.185
43.18966E-060.37
64.78448E-060.555
86.37931E-060.74

Image:R6G calibration curve.jpg

  • The concentration of the original stock solution was 398.7μM

Table 2: Actual concentration of dye in eppendorf samples

Sample number believed concentration Concentration in 1mL solution (uM) molar concentration in epp tubes (M)
12015.948275861.59483E-05
24031.896551723.18966E-05
36047.844827594.78448E-05
48063.793103456.37931E-05
510079.741379317.97414E-05
6150119.6120690.000119612
7200159.48275860.000159483

Notes

-These samples were left for too long without being tested and resulted in some of the dye releasing out of the complexes. For this reason, UV-Vis of these samples were not fully run and new samples were made.



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