User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/09/16: Difference between revisions
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== | ==September 16, 2014== | ||
* | ==Stock Solution Preparation== | ||
A 500 mL stock solution of 50 mM NaCl was made. | |||
*1.461 g of solid NaCl was dissolved in a 500 mL volumetric flask | |||
'''NOTE''': The purpose of this solution was to serve as a dialyzing agent | |||
A 1 L stock buffer solution of 50 mM glycine with a pH of approx. 3.5 (pKa in glycine buffer range) was made. | |||
*3.7576 g of glycine was dissolved in a 1 L volumetric flask with the addition of 4.00 mL of 1 M HCl | |||
'''NOTE''': The purpose of this solution was also to serve as a dialyzing agent. A buffer solution was necessary in order to change the ionic strength of the solution without altering its pH. | |||
**Pure water results in the precipitation of the AuNP colloid; an acidic buffer solution is conducive to maintaining the desired structure in a desired pH range while allowing for changes in ionic strength/conductivity and the movement of ions across the dialysis tubing | |||
A 25 mL solution of 1 g/L lysozyme was made. | |||
*0.025 g of lysozyme was dissolved in 25.0 mL of water | |||
'''NOTE''': The purpose of this solution was to serve as a baseline for the measurement of the equilibria/movement of free protein and/or ions across the dialysis tubing. | |||
A 10.0 mL sample of approximately 30:1 (rather 36.5:1) gold:protein colloid solution was obtained from previous experiments. | |||
*The sample came from the addition of 1 mL of 70.96μm lysozyme and 1 mL of 2.53 mM gold, and 8 mL of water in day one of lab - not included in lab notebook. | |||
==Dialysis== | |||
==Calibration Curves: pH and Conductivity== | |||
Revision as of 18:21, 16 September 2014
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September 16, 2014Stock Solution PreparationA 500 mL stock solution of 50 mM NaCl was made.
NOTE: The purpose of this solution was to serve as a dialyzing agent A 1 L stock buffer solution of 50 mM glycine with a pH of approx. 3.5 (pKa in glycine buffer range) was made.
NOTE: The purpose of this solution was also to serve as a dialyzing agent. A buffer solution was necessary in order to change the ionic strength of the solution without altering its pH.
A 25 mL solution of 1 g/L lysozyme was made.
NOTE: The purpose of this solution was to serve as a baseline for the measurement of the equilibria/movement of free protein and/or ions across the dialysis tubing. A 10.0 mL sample of approximately 30:1 (rather 36.5:1) gold:protein colloid solution was obtained from previous experiments.
DialysisCalibration Curves: pH and Conductivity | |
