User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/09/17

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September 17, 2014

Calibration Curves for pH and Conductivity Continued

The remaining pH and conductivity measurements for serial dilutions 50x, 100x, 200x, 500x of the 50 mM NaCl solution, as well as for all 8 serial dilutions of the 50 mM glycine solution, were found:

Concentration of NaCl (mM) pH Conductivity (μS/cm)
50 8.827 5560
25 8.357 2924
10 8.199 1173
5 8.075 638
2.5 7.873 315
1.0 8.248 148.5
0.5 7.474 76.6
0.25 7.263 424
0.1 7.147 23.62

The following graphs demonstrate the collected data:

Concentration of Glycine (mM) pH Conductivity (μS/cm)
50 3.492 543
25 3.540 330
10 3.646 177.8
5 3.774 111.2
2.5 3.967 66.1
1.0 4.283 35.6
0.5 4.565 19.20
0.25 4.846 13.57
0.1 5.486 10.21

The following graphs demonstrate the collected data:

Baseline pH and conductivity measurements for 1g/L lysozyme stock solution pre-dialysis

Concentration of Lysozyme (g/L) pH Conductivity (μS/cm)
1 5.700 144.6
1:6 dilution 6.123 24.14

Electrophoresis Sample Preparation

5 samples were prepared and stored in the fridge for electrophoresis next week.

  • 20 μL of the 1g/1L lysozyme stock solution were added to a 2 mL volumetric flask. 10 μL of this dilution were added to 10 μL of a running buffer prepared by Dr. Fox
  • 20 μL of the 36.5:1 [Au]:[Lys] colloid solution were added to a 2 mL volumetric flask. 10 μL of this dilution were added to 10 μL of the running buffer
  • 20 μL of a ___:__ [Au]:[BSA] colloid solution created the first day of lab were added to a 2 mL volumetric flask. 10 μL of this dilution were added to 10 μL of the running buffer
  • 20 μL of a 1g/1L BSA stock solution were added to a 2 mL volumetric flask. 10 μL of this dilution were added to 10 μL of the running buffer
  • 0.00209 g of powdered soy protein were dissolved in a 2 mL volumetric flask. 50 μL of this stock soy solution were then added to a 5 mL volumetric flask. 10 μL of this dilution were then added to 10 μL of the running buffer

Dialysis Sample Storage

The five dialysis samples prepared yesterday "soaked" overnight. Consequently, today they were removed from their respective soaks & tubing and placed in Falcon tubes for storage during the weekend, to be analyzed via Bradford assay next week.

NOTE: The lysozyme stock sample in 25K MWCO dialysis tubing (glycine soak) was spilled during transfer. The data for this sample will be obtained from another group.


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