User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/09/16: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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5 dialysis experiments were set up: | 5 dialysis experiments were set up: | ||
I | I - 4.0 mL of the prepared lysozyme solution was dialyzed in about 3 in of 3500 g/mol MWCO tubing in a 150 mL beaker filled with approx. 120 mL of the 50 mM NaCl stock solution. | ||
4.0 mL of the prepared lysozyme solution was dialyzed in about 3 in of 3500 g/mol MWCO tubing in a 150 mL beaker filled with approx. 120 mL of the 50 mM NaCl stock solution. | |||
II | II - 4.0 mL of the prepared lysozyme solution was dialyzed in about 3 in of 3500 g/mol MWCO tubing in a 150 mL beaker filled with approx. 120 mL of the 50 mM glycine stock solution. | ||
4.0 mL of the prepared lysozyme solution was dialyzed in about 3 in of 3500 g/mol MWCO tubing in a 150 mL beaker filled with approx. 120 mL of the 50 mM glycine stock solution. | |||
III | III - 4.0 mL of the prepared lysozyme solution was dialyzed in about 3 in of 3500 g/mol MWCO tubing in a 150 mL beaker filled with approx. 120 mL of pure water. | ||
4.0 mL of the prepared lysozyme solution was dialyzed in about 3 in of 3500 g/mol MWCO tubing in a 150 mL beaker filled with approx. 120 mL of pure water. | |||
IV | IV - 4.0 mL of the prepared lysozyme solution was dialyzed in about 3 in of 25k g/mol MWCO tubing in a 150 mL beaker filled with approx. 120 mL of the 50 mM glycine stock solution. | ||
4.0 mL of the prepared lysozyme solution was dialyzed in about 3 in of 25k g/mol MWCO tubing in a 150 mL beaker filled with approx. 120 mL of the 50 mM glycine stock solution. | |||
V | V - 4.0 mL of the 35.6:1 [Au]:[lys] solution wad dialyzed in about 3 in of 3500 g/mol MWCO tubing in a 150 mL beaker filled with approx. 120 mL of the 50 mM glycine stock solution. | ||
4.0 mL of the 35.6:1 [Au]:[lys] solution wad dialyzed in about 3 in of 3500 g/mol MWCO tubing in a 150 mL beaker filled with approx. 120 mL of the 50 mM glycine stock solution. | |||
NOTE: A sixth dialysis using 15k g/mol MWCO tubing was intended (4.0 mL of lysozyme solution against 50 mM glycine soln), but the specific tubing was not available. | '''NOTE''': A sixth dialysis using 15k g/mol MWCO tubing was intended (4.0 mL of lysozyme solution against 50 mM glycine soln), but the specific tubing was not available. | ||
==Calibration Curves: pH and Conductivity== | ==Calibration Curves: pH and Conductivity== | ||
pH and conductivity measurements were obtained for the baseline, as well as 4 serial dilutions (2x, 5x, 10x, 20x) of the 50 mM NaCl solution. | |||
==<font color=#0f1028></font>== | |||
{|style="width:700px" | |||
|<u>'''Concentration (mM)'''</u> | |||
|<u>'''pH'''</u> | |||
|<u>'''Conductivity (μS/cm)'''</u> | |||
|- | |||
|50 | |||
|8.827 | |||
|5.56 (mS/cm) | |||
|- | |||
|25 | |||
|8.357 | |||
|2924 | |||
|- | |||
|10 | |||
|8.199 | |||
|1173 | |||
|- | |||
|5 | |||
|8.075 | |||
|638 | |||
|- | |||
|2.5 | |||
|7.873 | |||
|315 | |||
|} | |||
'''NOTE''': To be continued... | |||
Latest revision as of 00:17, 27 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||||||||||
September 16, 2014Stock Solution PreparationA 500 mL stock solution of 50 mM NaCl was made.
NOTE: The purpose of this solution was to serve as a dialyzing agent A 1 L stock buffer solution of 50 mM glycine with a pH of approx. 3.5 (pKa in glycine buffer range) was made.
NOTE: The purpose of this solution was also to serve as a dialyzing agent. A buffer solution was necessary in order to change the ionic strength of the solution without altering its pH.
A 25 mL solution of 1 g/L lysozyme was made.
NOTE: The purpose of this solution was to serve as a baseline for the measurement of the equilibria/movement of free protein and/or ions across the dialysis tubing. A 10.0 mL sample of approximately 30:1 (rather 36.5:1) gold:protein colloid solution was obtained from previous experiments.
Dialysis5 dialysis experiments were set up: I - 4.0 mL of the prepared lysozyme solution was dialyzed in about 3 in of 3500 g/mol MWCO tubing in a 150 mL beaker filled with approx. 120 mL of the 50 mM NaCl stock solution. II - 4.0 mL of the prepared lysozyme solution was dialyzed in about 3 in of 3500 g/mol MWCO tubing in a 150 mL beaker filled with approx. 120 mL of the 50 mM glycine stock solution. III - 4.0 mL of the prepared lysozyme solution was dialyzed in about 3 in of 3500 g/mol MWCO tubing in a 150 mL beaker filled with approx. 120 mL of pure water. IV - 4.0 mL of the prepared lysozyme solution was dialyzed in about 3 in of 25k g/mol MWCO tubing in a 150 mL beaker filled with approx. 120 mL of the 50 mM glycine stock solution. V - 4.0 mL of the 35.6:1 [Au]:[lys] solution wad dialyzed in about 3 in of 3500 g/mol MWCO tubing in a 150 mL beaker filled with approx. 120 mL of the 50 mM glycine stock solution. NOTE: A sixth dialysis using 15k g/mol MWCO tubing was intended (4.0 mL of lysozyme solution against 50 mM glycine soln), but the specific tubing was not available. Calibration Curves: pH and ConductivitypH and conductivity measurements were obtained for the baseline, as well as 4 serial dilutions (2x, 5x, 10x, 20x) of the 50 mM NaCl solution.
NOTE: To be continued...
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