User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/08/27

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Preparation of Stock Solutions & Culture Tubes

We made 50 mL of a 2.512 mM solution of HAuCl4.

  • 0.043 g of solid HAuCl4 in 50 mL volumetric flask

We then made 50 mL of a 250 μM solution of lysozyme.

  • 0.179 g of solid lysozyme in 50 mL volumetric flask

6 culture tubes were prepared using the stock solutions to observe colloid versus fiber formation given different ratios of [Au]:[protein]. The table below demonstrates the amounts of each stock solution and water added to 6 test tubes, each containing 5 mL of solution total.


NOTE: The amount of Au in each tube was intended to be 0.5 mL, or 1/10 the volume of the final solution. Using a stock solution with a [H+] of 2.512 mM, the concentration of [H+] in the final 5 mL solution would be 2.512 x 10-4. This would give a solution of 3.6. Because we used 2.5 mL of Au in each tube, or 1/2 the volume of the final solution, our culture tubes contained a higher [H+] and therefore had a lower pH of 2.9.

Each test tube was covered in foil and placed in an oven at 80°C for 3 hours.
Au Lysozyme H2O [Au]:[lysozyme]
2.5 mL 2.5 mL 0 mL 10:1
2.5 mL 1.25 mL 1.25 mL 20:1
2.5 mL 0.835 mL 1.665 mL 30:1
2.5 mL 0.625 mL 1.875 mL 40:1
2.5 mL 0.5 mL 2 mL 50:1
2.5 mL 0.415 mL 2.08 mL 60:1


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Monika_Gasiorek/Notebook/CHEM-571_2014F/2014/08/27&oldid=812134"