User:Moira M. Esson/Notebook/CHEM-581/2013/02/06: Difference between revisions
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##cold water | ##cold water | ||
##methanol | ##methanol | ||
# | # Scrap all collected microspheres into a small vial. | ||
# Freeze microspheres in liquid nitrogen for ~2 minutes. | |||
# Place on [ask Dr. Hartings name of machine again] overnight to completely dry. | |||
*The following procedure was adapted from the article Poly(vinyl alcohol) microspheres with pH- and thermosensitive properties as temperature-controlled drug delivery [http://www.sciencedirect.com/science/article/pii/S1742706110002151] | *The following procedure was adapted from the article Poly(vinyl alcohol) microspheres with pH- and thermosensitive properties as temperature-controlled drug delivery [http://www.sciencedirect.com/science/article/pii/S1742706110002151] | ||
<br> | <br> | ||
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| Amount of H2SO4 added(mL)||0.25 | | Amount of H2SO4 added(mL)||0.25 | ||
|} | |} | ||
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Observations: | Observations: | ||
*The dispersion agent(hereout referred to as CAB), lamponite clay and PVA formed a sort of suspension in the water/organic solvent solution. It did not appear to fully dissolve. Used a spatula to scrape the side of the RB flask in order to try and break up the large chunks. | *The dispersion agent(hereout referred to as CAB), lamponite clay and PVA formed a sort of suspension in the water/organic solvent solution. It did not appear to fully dissolve. Used a spatula to scrape the side of the RB flask in order to try and break up the large chunks. | ||
* After addition to heat, the large chunks generally broke apart. Small spheres seemed to be forming after approximately an hour spinning. | * After addition to heat, the large chunks generally broke apart. Small spheres seemed to be forming after approximately an hour spinning. | ||
*After 3 hour spinning and heat, large clumps of spheres formed. Upon scraping with a spatula these clumps broke apart into what appeared to be smaller spheres. | |||
*Large clumps indicates that we may want to change portion of our method to produce smaller microspheres. | |||
*The spheres were a cloudy, white color throughout. | |||
==Hydrogel Preparation== | ==Hydrogel Preparation== |
Revision as of 12:39, 6 February 2013
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Objectives
PVA MicrospheresGeneral Protocol:
Hydrogel Preparation
Clay Filtration |