Objectives
- Remake hydrogels prepared on 2013/01/30 with dye added to the reaction mixture.
- Finish preparation of hydrogels with PVOH MW 146,000-186,000.
- Prepare exchanged clays.
Hydrogel preparation
- The following hydrogels were prepared following the general protocol described on 2013/01/30. However, the necessary amount of dye was added to each sample prior to freezing using the prepared DMSO/dye stock solution.
| Prepared Clay-PVA(MW 130,000) hydrogels
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| Composition of hydrogel(ratio of PVA to clay) | Amount of PVA added(g) | Amount of clay added(g) | Concencentration of DMSO/dye stock solution added(μM) | Amount of DMSO/dye added(mL)
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| 90:10 PVA:50% Bu3HdP-NaMT | 0.92966 | 0.0999 | 92 | 0.0655
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| 50:50 PVA:Bu3HdP-NaMT | 0.5061 | 0.5001 | 165 | 0.0364
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| 90:10 PVA:Lamponite | 0.9161 | 0.1004 | 92 | 0.0655
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| 50:50 PVA:Lamponite | 0.499 | 0.5009 | 165 | 0.0364
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| 90:10 PVA:NaMT | 0.8991 | 0.1002 | 92 | 0.0655
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| 50:50 PVA:NaMT | 0.5007 | 0.4996 | 165 | 0.0364
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| 90:10 PVA:110% Lamponite | 0.9008 | 0.0999 | 92 | 0.0655
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| 50:50 PVA:110% Lamponite | 0.4998 | | 165 | 0.0364
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| 90:10 PVA:110% NaMT | 0.8997 | 0.1005 | 92 | 0.0655
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Observations
- In order to have a complete and even spread of dye, a glass stirring rod was used to mix the hydrogels.
- Before addition of DMSO/dye, the hydrogels were allowed to cool slightly.
- Hydrogels with NaMT added did not have as even of a distribution of dye. Upon addition, the dye seemed to sit on top of the hydrogel. Extra stirring was required for these hydrogels.
Clay Preparation
- Surfactant clay exchanges were performed to create a 50% exchanged clay between NaMT and Bu3HdP and 110% exchange between NaMT and Lamponite.
- The general protocol for the preparation of 50% exchanged NaMT described on 2012/09/12 was followed.
- For the preparation of 110% exchanged NaMT and 110% exchanged Lamponite please refer to Karlena Brown's lab notebook.
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Project name
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