User:Moira M. Esson/Notebook/CHEM-571/2013/11/05: Difference between revisions

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==Notes==
*Lysozyme solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/30|10/30/2013]] all produced fibers and appear colorless except for the 30:1 ratio. This solution is mildly colored,
*All myoglobin solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/30|10/30/2013]] produced fibers and appear colorless. 




Revision as of 14:53, 11 November 2013

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Objectives


AA spectroscopy

General Protocol:

  1. Transfer ~4mL of sample from test tubes into a 10mL falcon tube.
  2. Centrifuge ~4mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced.
  3. Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL) following protocol described on 09/10/2013.
  4. Run samples on AA.
  5. Prepare calibration curve and prepare a graph showing the concentration of Au vs Au:Protein ratio


Table 1. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution

Concentration(μg/mL) Volume of stock Au solution(mL) Volume Deionized water added(mL)
10 0.1 9.9
20 0.2 9.8
30 0.3 9.7
40 0.4 9.6
50 0.5 9.5


Notes

  • Lysozyme solutions prepared on 10/30/2013 all produced fibers and appear colorless except for the 30:1 ratio. This solution is mildly colored,
  • All myoglobin solutions prepared on 10/30/2013 produced fibers and appear colorless.




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