User:Moira M. Esson/Notebook/CHEM-571/2013/10/30

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Objectives

  1. Synthesize new Myoglobin-AuNP solutions with different ratios in order to determine the optimal concentration of Au to protein for the formation of fibers
  2. Synthesize Lysozyme-AuNP solutions


Myoglobin-AuNP and Lysozyme-AuNP synthesis

  • The general protocol for the synthesis of HRP-AuNP and Pepsin-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on 08/28/2013.
  • 5mL of each solution was prepared.


Table 1. Preparation of Myoglobin-AuNP solutions

Ratio Myoglobin Amount of gold added(mL) Concentration Myoglobin(M) Volume Myoglobin(mL) Volume Water Myoglobin(mL)
50.4942645580.0000522.505735442
100.4942645580.00002513.505735442
150.4942645581.66667E-050.6666666673.839068775
200.4942645580.00001250.54.005735442
250.4942645580.000010.44.105735442
300.4942645588.33333E-060.3333333334.172402108
350.4942645587.14286E-060.2857142864.220021156
400.4942645580.000006250.254.255735442
450.4942645585.55556E-060.2222222224.28351322
500.4942645580.0000050.24.305735442


Table 2. Preparation of all Lysozyme-AuNP solutions

Ratio Lysozyme Amount gold added (mL) Concentration Lysozyme(M) Volume Lysozyme (mL) Volume Water Lysozyme (mL)
300.4942645588.33333E-061.6111486492.894586793
500.4942645580.0000050.9666891893.539046253
700.4942645583.57143E-060.6904922783.815243164
900.4942645582.77778E-060.537049553.968685892
1100.4942645582.27273E-060.4394041774.066331265
1300.4942645581.92308E-060.3718035344.133931907
1500.4942645581.66667E-060.322229734.183505712
1700.4942645581.47059E-060.284320354.221415092
1900.4942645581.31579E-060.2543918924.25134355
2100.4942645581.19048E-060.2301640934.275571349


Table 3. Stock Solution concentrations

Concentration of Myoglobin (M) Concentration Gold(M) Concentration Lysozyme (M)
0.0001250.002529012.58615E-05
  • All stock solutions were prepared with deionized H2O
  • The gold stock solution was prepared using Gold Chloride (II) trihydrate


Table 4. Preparation of Lysozyme Stock solution

Molecular Weight(g/mol) 14307
mass lysozyme(g)0.0037
volume solution(mL)10
Concentration Lysozyme (M)2.58615E-05


Table 5. Preparation of Myoglobin Stock solution

Molecular weight (g/mol) 17600
mass myoglobin (g)0.022
volume solution (mL)0.01
Concentration (M)0.000125


Notes

  • All of the prepared AuNP solutions had a slight yellow tinge to the solutions (from the presence of the gold).
  • All of the prepared AuNP solutions will be tested using UV/vis and AA spectroscopy




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