User:Moira M. Esson/Notebook/CHEM-571/2013/10/09

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(Kinetics)
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*A new solution of 40μM adenosine solution was prepared following the protocol described on [[User:Moira_M._Esson/Notebook/CHEM-571/2013/10/08|2013/10/08]].
*A new solution of 40μM adenosine solution was prepared following the protocol described on [[User:Moira_M._Esson/Notebook/CHEM-571/2013/10/08|2013/10/08]].
*2μL EHNA was added to the prepared adenosine solution for approximately 1 minute before placing the solution in the cuvette.
*2μL EHNA was added to the prepared adenosine solution for approximately 1 minute before placing the solution in the cuvette.
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*A more detailed protocol    
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*A more detailed protocol is described in [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/10/08|Matt Harting's]] notebook
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<br>
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Prepared solutions:
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*Note: The following solution was prepared by [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/10/08|Matt Harting's]]
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<br>
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EHNA stock
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5mg EHNA in 1mL DMSO ---> 15.9mM EHNA
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(1.9μL)(15.9mM EHNA)=(10mL)(C2). C2 ---> 3μM EHNA
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The reaction samples will contain roughly 1nM EHNA
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<br>
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'''UV/vis'''     
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Revision as of 21:57, 12 October 2013

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Objectives

  1. Measure the kinetics of the conversion of adenosine to inosine using ADA as a catalyst, in the presence of the inhibitor EHNA.


Kinetics

General Protocol:

  • The general protocol followed the general protocol described on 2013/10/08.
  • A new solution of 40μM adenosine solution was prepared following the protocol described on 2013/10/08.
  • 2μL EHNA was added to the prepared adenosine solution for approximately 1 minute before placing the solution in the cuvette.
  • A more detailed protocol is described in Matt Harting's notebook


Prepared solutions:


EHNA stock 5mg EHNA in 1mL DMSO ---> 15.9mM EHNA (1.9μL)(15.9mM EHNA)=(10mL)(C2). C2 ---> 3μM EHNA The reaction samples will contain roughly 1nM EHNA
UV/vis


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