User:Michael S. Bible/Notebook/CHEM-671/690 Lab Notebook/2015/09/02: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav)
 
(2 intermediate revisions by 2 users not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
Line 29: Line 29:
These solutions were then heated in the oven at 80°C for four hours.   
These solutions were then heated in the oven at 80°C for four hours.   


 
==Results==
* All ten 5 mL solutions failed to produce nanofibers.
* All sixteen 1 mL solutions failed to produce nanofibers. 
* All solutions that failed to produce nanofibers yielded solutions of nanoparticles. 
* Solutions of nanoparticles created by accident were stored in a 50 mL falcon tube should a need for them arise in the future of our study. 




Latest revision as of 01:07, 27 September 2017

Project name Main project page
Next entry

Objective

Prepare solutions of lysozyme/AuNP fibers.

Procedure

Stock solutions of HAuCl4·H2O and Lysozyme were prepared by Dr. Hartings. The procedure for their preparation can be found here.

The stock solution of HAuCl4·H2O has a concentration of 2.96 mM. The stock solution of Lysozyme has a concentration of 21.4 μM.

Sixteen 1 mL samples and ten 5 mL samples of a 45:1 Au:Lysozyme solution were prepared using the following volumes of stock solutions:

  1. 1 mL samples
    1. Volume of gold stock: 84.4 μL
    2. Volume of protein stock: 259 μL
    3. Volume of water: 656 μL
  2. 5 mL samples
    1. Volume of gold stock: 422 μL
    2. Volume of protein stock: 1,297 μL
    3. Volume of water: 3,281 μL

These solutions were then heated in the oven at 80°C for four hours.

Results

  • All ten 5 mL solutions failed to produce nanofibers.
  • All sixteen 1 mL solutions failed to produce nanofibers.
  • All solutions that failed to produce nanofibers yielded solutions of nanoparticles.
  • Solutions of nanoparticles created by accident were stored in a 50 mL falcon tube should a need for them arise in the future of our study.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Michael_S._Bible/Notebook/CHEM-671/690_Lab_Notebook/2015/09/02&oldid=1017156"