User:Michael S. Bible/Notebook/CHEM-671/690 Lab Notebook/2015/09/02

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Objective

Prepare solutions of lysozyme/AuNP fibers.

Procedure

Stock solutions of HAuCl4·H2O and Lysozyme were prepared by Dr. Hartings. The procedure for their preparation can be found here.

The stock solution of HAuCl4·H2O has a concentration of 2.96 mM. The stock solution of Lysozyme has a concentration of 21.4 μM.

Sixteen 1 mL samples and ten 5 mL samples of a 45:1 Au:Lysozyme solution were prepared using the following volumes of stock solutions:

  1. 1 mL samples
    1. Volume of gold stock: 84.4 μL
    2. Volume of protein stock: 259 μL
    3. Volume of water: 656 μL
  2. 5 mL samples
    1. Volume of gold stock: 422 μL
    2. Volume of protein stock: 1,297 μL
    3. Volume of water: 3,281 μL

These solutions were then heated in the oven at 80°C for four hours.

Results

  • All ten 5 mL solutions failed to produce nanofibers.
  • All sixteen 1 mL solutions failed to produce nanofibers.
  • All solutions that failed to produce nanofibers yielded solutions of nanoparticles.
  • Solutions of nanoparticles created by accident were stored in a 50 mL falcon tube should a need for them arise in the future of our study.



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