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Project name
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Objective
- Determine concentration of ADA prepared Oct. 23 via Bradford Assay
Bradford Assay
- A standard curve for UV/Vis was obtained with the following concentrations of BSA:
volume BSA (uL)
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volume H2O (uL
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[BSA] (mg/mL)
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[BSA] (M)
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0 |
30 |
0 |
0.00E+00
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5 |
25 |
0.24 |
3.61E-06
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10 |
20 |
0.15 |
2.26E-06
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15 |
15 |
0.13 |
2.01E-06
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20 |
10 |
0.15 |
2.26E-06
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25 |
5 |
0.24 |
3.61E-06
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30 |
0 |
1 |
1.51E-05
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- The ADA was analyzed via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration.
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