User:Michael F. Nagle/Notebook/Chem 571/2012/11/06: Difference between revisions
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==Procedure== | ==Procedure== | ||
*The prodedure for [AU_Biomaterials_Design_Lab:Protocols/PCR_Mutation_Protocol|PCR Mutation] was followed, with the following modifications | *The prodedure for [[AU_Biomaterials_Design_Lab:Protocols/PCR_Mutation_Protocol|PCR Mutation]] was followed, with the following modifications | ||
**20μL DNA solution was added to 30μL solution with E. Coli cells. | **20μL DNA solution was added to 30μL solution with E. Coli cells. | ||
**250μL Lysogeny Broth (LB) was used instead of SOC medium. | **250μL Lysogeny Broth (LB) was used instead of SOC medium. | ||
**Single 200μL plates were prepared. No 50μL plates were made. | **Single 200μL plates were prepared. No 50μL plates were made. | ||
*Adenosine Deaminase prepared [User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23] was purified via FPLC | *Adenosine Deaminase prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] was purified via FPLC | ||
** Pump flow was 5mL/minute | ** Pump flow was 5mL/minute | ||
Revision as of 11:32, 7 November 2012
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Objectives
Procedure
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