User:Matthewmeisel/Notebook/2006-6-13

DNA nanostructure reaction PCR

• order of lanes: 1) 1kb ladder, 2) full rxn (oligos + scaffold), 3) just scaffold, 4) just oligos
• run on 2% agarose gel
• appears that reaction was successful: gel image (leftmost four lanes) shows expected results
  • assembled nanostructure runs slightly faster than scaffold, oligos appear as a smear of short DNAs

Transformation of standard components

• results of plated bacteria: all three plates showed colony growth, negative control did not
• colony selection and amplification

1. Seven culture tubes were filled with 5 mL LB medium and 50 μl of 5 mg/mL ampicillin
2. Colonies were selected from the three plates (three from R0010, two from E7104, two from E0241) and were transferred into respective culture tubes with a sterilized pick
3. Culture tubes were incubated, with shaking, at 37°C for 18 h.
4. Plates were stored at 4°C.