User:Mar/Notebook/2007-5-24

test IHC on vibratome HET sections

used combinations of lab's Abs - 200µL/well (i.e. 400µL of each comb.)

No.	mouse	rabbit
1.	p73 [?] 4µL	Stat3 [10µg/mL = 1:1000] 4µL
2.	HRG Ab-1 [1:25-1:50] 16µL	Sox2 [1:1000] 0.4µL
3.	TGFalpha (134A)[1:50-1:500] 2µL	BDNF [1:50-1:500] 4µL
4.	eIF4E [?] 4µL	TGFalpha (H50) [1:50-1:500] 2µL
5.	PKAc [?] 4µL	ErbB3 [1:50-1:500] 2µL

primary (in PBS + 0.1% Triton + 10% NGS) 250µL per 1/24 well (excess)
o/n 4°C shaking