User:Mar/Notebook/2007-5-24

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test IHC on vibratome HET sections

used combinations of lab's Abs - 200µL/well (i.e. 400µL of each comb.)
No. mouse rabbit
1. p73 [?] 4µL Stat3 [10µg/mL = 1:1000] 4µL
2. HRG Ab-1 [1:25-1:50] 16µL Sox2 [1:1000] 0.4µL
3. TGFalpha (134A)[1:50-1:500] 2µL BDNF [1:50-1:500] 4µL
4. eIF4E [?] 4µL TGFalpha (H50) [1:50-1:500] 2µL
5. PKAc [?] 4µL ErbB3 [1:50-1:500] 2µL
primary (in PBS + 0.1% Triton + 10% NGS) 250µL per 1/24 well (excess)
o/n 4°C shaking
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