User:Madeline Hartman/Notebook/Dr. Hartings Lab/2013/02/05: Difference between revisions
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==Entry title== | ==Entry title== | ||
Run PCR of Forward/Reverse DNA product | |||
Make end total of 4 tubes- 8 tubes combined into 4 | |||
PCR Master mix contains- Buffer, enzyme, dNTPs | |||
Each tube- 25 μL- 12.5 μL master mix, acid primer, DNA | |||
Revision as of 12:16, 22 September 2013
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Entry titleRun PCR of Forward/Reverse DNA product Make end total of 4 tubes- 8 tubes combined into 4 PCR Master mix contains- Buffer, enzyme, dNTPs Each tube- 25 μL- 12.5 μL master mix, acid primer, DNA
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