User:Madeline Hartman/Notebook/Dr. Hartings Lab/2013/01/29

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Gel electrophoresis was run to test PCR

1% Agarose Gel- .25 g agarose

                               25 mL electrophorsis buffer

Microwave for 45 seconds to dissolve

Lane 2- 6 μL marker

Lane 3- 1 μL dye + 5 μL original DNA

Lane 4- 1 μL dye + 5μL forward/reverse DNA

Run at 100 V for 60 minutes

Results:

Original- No Band

Forward/Reverse- Band



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