User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/05: Difference between revisions
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===FCS Sample Prep=== | ===FCS Sample Prep=== | ||
*Green fluorescent beads | *Green fluorescent beads | ||
**10000x diluted | **10000x diluted, run 3x for 90s | ||
*PBS | |||
**To check for background, run 3x for 200s | |||
*DNA/MB | *DNA/MB | ||
**125/100pM | **125/100pM, run 3x for 500s | ||
**100/80pM | **100/80pM, run 3x for 500s | ||
**75/60pM | **75/60pM, run 3x for 500s | ||
**50/40pM | **50/40pM, run 3x for 500s | ||
**25/20pM | **25/20pM, run 3x for 500s | ||
===FCS Data=== | ===FCS Data=== | ||
Graphs generated using Igor Pro.<br.> | |||
<br.> | |||
[[Image:Avg_Beads.png]]<br.> | |||
10000x diluted green fluorescent beads were used to align the laser. Sample was run three times, for 90s each.<br.> | |||
<br.> | |||
[[Image:Avg_PBS.png]]<br.> | |||
PBS run to check for background fluorescence. Sample was run three times, 200s each to eliminate noise.<br.> | |||
[[Image:Avg_125pM.png]]<br.> | |||
<br.> | |||
[[Image:Avg_100pM.png]]<br.> | |||
<br.> | |||
[[Image:Avg_75pM.png]]<br.> | |||
<br.> | |||
[[Image:Avg_50pM.png]]<br.> | |||
<br.> | |||
[[Image:Avg_25pM.png]]<br.> | |||
All DNA/MB samples run three times, for 500s.<br.> | |||
<br.> | |||
Calibration Graph:<br.> | |||
[[Image:2013_0705_calibration.PNG]] | |||
Revision as of 13:49, 5 July 2013
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July 5, 2013FCS Sample Prep
FCS DataGraphs generated using Igor Pro.<br.> <br.> <br.> 10000x diluted green fluorescent beads were used to align the laser. Sample was run three times, for 90s each.<br.> <br.> <br.> PBS run to check for background fluorescence. Sample was run three times, 200s each to eliminate noise.<br.> <br.> <br.> <br.> <br.> <br.> <br.> <br.> <br.> <br.> All DNA/MB samples run three times, for 500s.<br.> <br.> Calibration Graph:<br.>
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